Preservation of Physarum polycephalum plasmodia in liquid nitrogen
نویسندگان
چکیده
منابع مشابه
Post-fusion somatic incompatibility in plasmodia of Physarum polycephalum.
High-resolution autoradiography has been used to establish that during the incompatibility reaction that follows fusion between plasmodia of a 'killer' and a 'sensitive' strain of the myxomycete Physarum polycephalum, the nuclei of the sensitive strain are selectively damaged, enclosed in vacuoles and eliminated from the cytoplasm. This damage is visible as increased chromatin condensation and ...
متن کاملPost-fusion Incompatibility in Plasmodia of Physarum Polycephalum
In the myxomycete Physarum polycephalum the somatic incompatibility between plasmodia of a killer strain and a sensitive strain is expressed as a lethal reaction (LR) in the sensitive strain within 6 h after fusion of both plasmodia. The lethal reaction was not caused by any of the extra-cellular compounds produced by the myxomycete nor was the result of intra-cellular compounds of the killer s...
متن کاملGrowth of large plasmodia of the myxomycete Physarum polycephalum.
A method has been developed for growing Physarum polycephalum plasmodia that are 8 to 10 times larger than those obtained in the petri dish cultures used by Nygaard, Guttes, and Rusch. In the large-scale procedure, plasmodia were grown in metal trays on a membrane supported by filter paper on stainless-steel screen. Plasmodia were started from a ring of inoculum to allow inward and outward migr...
متن کاملSubcellular Localization of Calcium Repositories in Plasmodia of the Acellular Slime Mold Physarum Polycephalum
Ul t ras t ruc tura l fibrils consisting of densely packed microfilaments, s imdar in size to the th in filaments of striated muscle, have been observed in the p lasmodium of the acellular slime mold Ph)'sarum pol)'cephalum (Rhea, 1966; Wohl fa r th Bot termann, 1964) A prote in wi th sedimenta t ion characteristics and ATPase activity typical of muscle myosin is also extractable f rom shme mol...
متن کاملThe Changing Pattern of Birefringence in Plasmodia of the Slime Mold, Physarum Polycephalum
Plasmodia of the acellular slime mold, Physarum polycephalum, reveal a complex and changing pattern of birefringence when examined with a sensitive polarizing microscope. Positively birefringent fibrils are found throughout the ectoplasmic region of the plasmodium. In the larger strands they may be oriented parallel to the strand axis, or arranged circularly or spirally along the periphery of e...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1967
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.94.4.1257-.1967